Ph.D., University of Georgia

Associate Professor

Primary Affiliation:UT FST
Expertise: Molecular Biology


 

Professional Preparation

B.S. Microbiology/Biochemistry, St. Xavier's College, University of Mumbai

Ph.D. Food Science & Technology (Microbiology), The University of Georgia, Athens, GA.

Current Research: Rapid molecular detection of foodborne bacterial and viral pathogens; novel efficacious control and inactivation strategies for foodborne pathogens; survival, fate and transmission of foodborne pathogens in the environment


Appointments

Assistant Professor; Department of Food Science and Technology
University of Tennessee, Knoxville, TN

Research Scientist/Postdoctoral Research Associate
North Carolina State University, Raleigh, NC

Research Coordinator/Postdoctoral Research Associate
University of Georgia, Athens, GA



Publications

D’Souza, D.H., F.J. Critzer, and D.A. Golden. 2009. Real-time reverse-transcriptase-PCR (RT-PCR) for the rapid detection of Salmonella using invA primers. Foodborne Path. Dis. 6(9):1-10.

D’Souza, D. H., X. Su, A. Roach, and F. Harte. 2009. High pressure homogenization for the inactivation of human enteric virus surrogates. Journal of Food Protection 72:In Press

Stenske, K.A., D.A. Bemis, B.E. Gillespie, D.H. D’Souza, S.P. Oliver, F.A. Draughon, K.J. Matteson, and J.W. Bartges. 2009. Comparison of clonal relatedness and antimicrobial susceptibility of fecal Escherichia coli from healthy dogs and their owners. Amer. J. Veterinary Research 70:1108-1116.

 

 

Santos, F.B.O, D. H. D’Souza, L. Jaykus, P. R. Ferket and B.W. Sheldon. 2007. Genotypes, serotypes, and antibiotic resistance profiles of Salmonella isolated from commercial North Carolina turkey farms. Journal of Food Protection. 70(6):1328-1333.

D’Souza, D. H., A.Sair, K.Williams, E.Papafragkou,J. Jean,C.Moore and L.Jaykus.2006.Persistence of Norwalk virus on environmental surfaces and its transfer to food. International Journal of Food Microbiology 108(1):84-91.

Jean, J., D. D’Souza and L. Jaykus. 2004. Multiplex NASBA for the detection of hepatitis A and Norwalk viruses in foods. Applied and Environmental Microbiology 70: 6603-6610.

D’Souza , D. H. and L. A. Jaykus. 2003. NASBA for the rapid and sensitive detection of Salmonella enterica from foods. Journal of Applied Microbiology 95: 1343-1350.

D’Souza, D H. 2003. Methods for virus detection in foods. Journal of the Association for Food and Drug Officials, Invited Review paper, December issue. Vol. 4: 26-37.

Jean, J., D. D’Souza and L Jaykus. 2003. Transcriptional enhancement of RT-PCR for rapid and sensitive detection of enteric viruses. FEMS Microbiology Letters 226: 339-345.

D’Souza, D. H. and L.A. Jaykus. 2002. Zirconium hydroxide effectively immobilizes and concentrates human enteric viruses. Letters in Applied Microbiology 35(5): 414-418.

Sair, A. I., D. H. D’Souza, and L. A. Jaykus. 2002. Human enteric viruses as causes of foodborne diseases. Comprehensive Reviews in Food Science and Food Safety 1 (2): 73-89.

Sair, A. I., D. H. D’Souza, C. L. Moe, and L. A. Jaykus. 2002. Improved detection of human enteric viruses in foods by RT-PCR. Journal of Virological Methods 100 (1-2): 57-69.

D’Souza, D. H. and R. E. Brackett. 2001. Reducing conditions and seryl and sulfhydryl group inhibitors on aflatoxin degradation by Flavobacterium aurantiacum. Journal of Food Protection 64(2): 268-271.

D’Souza, D. H. and R. E. Brackett. 2000. The influence of divalent cations and chelators on aflatoxin B1 degradation by Flavobacterium aurantiacum. Journal of Food Protection 63(1): 102-105.

D’Souza, D. H. and R. E. Brackett. 1998. The role of trace metal ions in aflatoxin B1 degradation by Flavobacterium aurantiacum. Journal of Food Protection 61(12): 1666-1669.

BOOK CHAPTERS (total of 5)

D. H. D’Souza, C. L. Moe, L. Jaykus. 2007. Foodborne Viral Pathogens. Food Microbiology, Fundamentals and Frontiers. Eds, M.P. Doyle, L.R. Beuchat, ASM Press

D. H. D’Souza and L.A. Jaykus. 2006. Rapid Diagnostic Methods in Food Safety: Viruses. Submitted. Encyclopedia of Biotechnology in Agriculture and Food. Eds D. Heldman, A. Bridges, D. Hoover and M. Wheeler. Francis and Taylor group, LLC, New York, NY.

D. H. D’Souza and L. Jaykus. 2006. Molecular Approaches for the Detection of Foodborne Viral Pathogens. In PCR Methods in Foods. Ed. J. Maurer, Springer, New York, NY.

D. H. D’Souza, J. Jean and L. Jaykus. 2006. Methods for Detection of Viral and Parasitic Pathogens. In food. In: Handbook of Food Science, Technology and Engineering. Editors Hui, Y. H et al. (eds) CRC press, Boca Raton, FL.

E.Papafragkou, D. H. D’Souza and L. Jaykus. 2006. Foodborne Transmitted Viruses: Prevention and Control. Ed. S. Goyal, Springer, New York, NY.

SELECTED RECENT PRESENTATIONS (from a total of 45):

Su, X, S.Zivanovic, and D. H. D'Souza. The effect of chitosan against the infectivity of human enteric virus surrogates, IAFP, Grapevine, TX, 2009.

 

Techathuvanan, C., F. A. Draughon, D. H. D'Souza. Reverse-Transcriptase Loop-mediated Isothermal Amplification (RT-LAMP) for the Detection of Salmonella Typhimurium in Pork. ASM, Philadelphia, PA, May 2009.

Miller, N.D., P. M. Davidson, D. H. D'Souza. Rapid Detection of Salmonella Typhimurium from Spiked Lettuce and Tomatoes using Real-Time Reverse Transcriptase-Polymerase Chain Reaction. IAFP, Grapevine, TX, July, 2009.

Xiao, D., P.M. Davidson, J. Lin, D. D'Souza, Q. Zhong. Capacity of Aqueous Methanol and Ethanol to Extract Nisin from a 2.5% Commercial Preparation, IFT, Anaheim, CA, June, 2009.

Techathuvanan, C., F. A. Draughon. D. H. D'Souza. Real-time Reverse-Transcriptase Polymerase Chain Reaction for the rapid and sensitive detection of Salmonella Typhimurium from pork, IFT, Anaheim, CA, June, 2009.

Critzer, F. J., D.H. D’Souza. Construction of an internal amplification control by in vitro transcription for Salmonella detection using real-time RT-PCR. IAFP, Columbus, OH, August, 2008

Critzer, F.J, D. H. D’Souza, D. A. Golden. Expression of the urease operon in Escherichia coli O157:H7 treated with 0.5% sodium benzoate. IAFP oral presentation, Columbus, OH, August, 2008

Carter, K., D. H. D’Souza, A. Saxton and D.A. Golden. Transcriptome analysis of Escherichia coli O157:H7 under acidic conditions. IAFP oral presentation, Columbus, OH, August, 2008

Critzer, F.J, D. H. D’Souza, D. A. Golden. Expression of the Phosphate Specific Transport (Pst) System of Escherichia coli O157:H7 Treated with 0.5% Sodium Benzoate. ASM Meeting, Boston, MA, June, 2008.

Miller, N. D., D. H. D’Souza. Real-time RT-PCR for the rapid detection of Salmonella from lettuce. College of Experimental Medicine Symposium, UT-Knoxville, June 17, 2008.

D’Souza, D.H, F.J.Critzer, D.Golden. RT-PCR (real-time) for the rapid detection of Salmonella using invA primers. IAFP poster, Orlando, FL, July 10, 2007.

Critzer, F. J., D.H. D’Souza, D. Golden. Transcription analysis of stx1 and marA genes in Escherichia coli O157:H7 with sodium benzoate. IAFP, FL, July 2007.

Carter, K, J. Gouffon, D.H. D'Souza, D. Golden. Gene expression analysis of Escherichia coli O157:H7 at 10 and 30 C by use of high density oligonucleotide microarrays. IAFP poster, FL, July 2007.

 

Ouassa,T.,D.Golden, X. Anglaret, H.Menan. D. H. D’Souza. Antibiotic resistance profiles of Salmonella from HIV-1infected adults inAbidjan,Cote d’Ivorie.ASM Poster, Toronto, May 2007.

Escudero, B., D. H. D’Souza, and L. Jaykus. Rapid detection of multiple foodborne pathogens using a PCR-reverse dot blot hybridization approach. ASM Poster, Orlando, FL, May 22, 2006.

Park, J., D. H. D'Souza, P. Liu, C. L. Moe and L. Jaykus. Persistence of Noroviruses on food processing surfaces and human hands. ASM Poster, Orlando, FL, May 22, 2006.

Escudero, B.I., D. H. D’Souza, and L. Jaykus. Detection of an array of foodborne pathogens by use of universal primers and specific oligoprobes. Poster, IAFP, MD, Aug 2005.

Stam, C., D. H. D’Souza, S. Kathariou, P. Curtis,R. Meinersmann, L. Jaykus. Prevalence and persistence of foodborne pathogens in turkey processing environment. IAFP, MD, Aug 2005.



Honors and Awards/Grants and Contracts

 

2001 North Carolina State University Outstanding Research Award

1997 Developing Scientist Award, First Place Oral Competition, IAMFES (IAFP)

1997 E. M. Ahmed SAAS-IFT Graduate Paper Award

1996 & 1997 GA Association of Food & Environmental Sanitarians(GAFES)Young Investigator Award

1996 Dixie IFT Travel Award

1996 University of Georgia Travel Award

1996, Finalist-Broadus E. Browne Outstanding Ph.D. graduate student College of Agriculture and Environmental Sciences, University of Georgia, Athens

1993-1997 US Agency for International Development and Peanut Collaborative Research Support Group Funding for Ph.D. research

1994-1995 Student Representative-Dixie IFT Section

1991-1993 Phi Beta Delta, UGA-Honor Society for International Scholars

First Rank Holder(Gold Medalist): Master's Progam(Microbiology), Univ. of Mumbai

Govt.of India Open Merit Scholarship for Master's Progam, Univ. Mumbai

Govt.Open Merit Scholarship for all 3 years of Bachelors Progam, Univ. Mumbai



Synergistic Activities

Member: American Society for Microbiology, International Association for Food Protection, IFT

Editorial Board: Journal of Food Protection; Food Analytical Methods;
Applied and Environmental Microbiology
Ad-hoc Reviewer: Food Microbiology

Chair (2007-2008): Viral and Parasitic Foodborne Disease Professional Development Group, IAFP; Vice-Chair (2005-2006).

Co-Faculty Advisor (2007-2009): Food Science Club, University of Tennessee-Knoxville

Advisor: 1 Ph.D. Student (Chayapa Techathuvanan); 1 M.S.Student (Nathan Miller-graduated Spring 2009); 1 undergrad intern (Glafir Hernandez-Summer 2008; Luz Meneses-Summer 2009);

Co-advisor: 1 Ph.D. Student (Faith J. Critzer) (graduated Spring 2008)

Supervisor: 1 Research Associate: Xiaowei Su
Co-supervisor: 1 Visiting Scientist (Dr. T. Ouassa: Fulbright Scholar with Dr. Golden, Spring-Summer-Fall 2007)

Committee Member: 2 Ph.D. Students (Kristina Carter, Carolina Naar)(graduated December 2007) 1: Ph.D. Student: Dan Xiao)


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Davidson 

Contact Dr. D'Souza

102 Food Safety & Processing Bldg.
2600 River Drive
Knoxville, TN 37996-4591
Phone: (865) 974-2753
Fax: (865) 974-7332
Email:ddsouza@utk.edu